山楂叶总黄酮清除DPPH和超氧阴离子自由基的活性研究

Study on DPPH and Superoxide Free Radical Scavenging Capacity of Total Flavonoids from Hawthorn Leaves

以山楂秋日落叶为原料,70%乙醇为溶剂,提取得到山楂叶总黄酮。以芦丁和VC为对照,采用分光光度法研究了山楂叶总黄酮对DPPH自由基和超氧阴离子自由基的体外清除活性。结果表明,山楂叶总黄酮对DPPH自由基和超氧阴离子自由基均具有一定的清除作用。对DPPH自由基的清除率随加入量的增加而增加,当加入量为2 mg时,清除率为50.29%;而对超氧阴离子自由基的清除在加入量为1.2 mg时达到最高值27.36%。芦丁对二自由基的最高清除率分别为63.83%和32.55%;而VC则分别为69.93%和99.04%。经比较发现,山楂叶总黄酮的清除自由基活性稍低于芦丁,远低于VC。

Take hawthorn fallen leaves in autumn as raw material to extract total flavonoids by 70% ethanol as solvent. Rutin and vitamin C were used as reference substances and DPPH and superoxide free radical scavenging capacities of total flavonoids from hawthorn leaves were studied by spectrophotometric method. The results showed that the total flavonoids have some scavenging effects on both DPPH and superoxide free radical. For DPPH free radical, the scavenging rate improves with the addition of the total flavonoids increasing. The scavenging rate was 50. 29% when the addition was up to 2mg. The superoxide free radical scavenging rate got to maximum value 27.36% when the addition was 1.2mg. The maximum values of rutin were 63.83% and 32.55% respectively. Furthermore, those of vitamin C were 69.93% and 99.04%. It is found by comparison that free radical scavenging capacities of the total tlavonoids had a little lower than those of rutin, however, significantly inferior to vitamin C.