CA/PEI亲和膜去除肝硬化腹水中内毒素的研究

Endotoxin removal in ascites of patients with cirrhosis by CA/PEI affinity membrane in vitro

目的观察CA/PEI亲和膜对肝硬化腹水中内毒素的去除效果。方法以醋酸纤维素(CA)为亲和基质,交联聚乙烯基亚胺(PEI),制备CA/PEI亲和膜;采用动态吸附法,比较吸附前后肝硬化患者腹水中内毒素、白蛋白、免疫球蛋白(IgG、IgA、IgM)及离子(K+、Na+、Cl-)浓度的变化。结果CA/PEI亲和膜吸附后腹水中的内毒素浓度显著降低(P<0.01);腹水中的白蛋白、免疫球蛋白、电解质浓度过膜前后无明显变化。结论CA/PEI亲和膜能有效吸附肝硬化腹水中的内毒素,对腹水中的白蛋白、免疫球蛋白、电解质无明显截留,可应用于肝硬化自体腹水回输的治疗。

Objective To investigate the removal of endotoxin from ascites of patients with cirrhosis by cellulose diacetate/polyethyleneimine affinity membrane in vitro. Methods The CA/PEI affinity membrane was prepared by employing cellulos diacetate as matrix immobilized polyethyleneimine as affinity ligand,and the dynamic absorption of ascites through CA/PEI affinity membrane was conducted. The concentration of endotoxin,albumin,immunoglobulin （IgG,IgA and IgM）and electrolytes（K＋,Na＋ and C1-）in the ascites before and after absorption were determined. Results The endotoxin level was greatly reduced （P〈0.01）after absorption. There was no markedly changes for the concentration of albumin,im- munoglobulin and electrolytes in the ascites. Conclusion CA/PEI affinity membrane can effectivly absorb the endotoxin from the ascites,and the absorption has no obviously influence on the albumin,immunoglobulin and electrolytes at the same time.