HepG2与HepG2.2.15细胞中P53表达及活性的比较

The expression and activity of P53 in HepG2 and HepG2.2.15 cells

目的探讨HBV对抑癌基因P53表达及活性的影响。方法选用HepG2及转染了HBV表达质粒的HepG2.2.15细胞,采用Western blotting法检测两种细胞P53的表达状况;磷酸钙法将报告基因质粒PG13-CAT和P21-LUC分别转染细胞,通过检测报告基因的表达观察各细胞中P53的活性。结果HepG2.2.15细胞中P53蛋白表达水平高于HepG2细胞,而且两种报告基因的表达在HepG2.2.15细胞中也较高。结论HBV在肝癌细胞内的复制对P53的表达及功能具有一定的增强作用。

Objective To investigate the effects of hepatitis B virus（HBV）on expression and activity of tumor suppressor gene P53 in hepatoma cell line. Methods Parental HBV-free HepG2 ceils and HBV-transfected HepG2.2.15 cells were selected. P53 expression level was evaluated by western blotting,and the P53 activity was indicated by reporter genes expression which were transfected by phosphate calcium precipitation. Results HepG2.2.15 ceils had a higher expression and activity of P53 than that in HepG2 cells. Conclusion Replication of HBV in hepatoma cells has some enhancing effect on P53 expression and activity.