慢性HBV感染者外周血单个核细胞CTLA-4基因多态性与病情转归的关联研究

Study on association between the single nucleotide polymorphism of cytotoxic T lymphocyte associated agent 4 gene exon 1 region and outcome of patients with hepatitis virus B infection

目的初步探讨慢性HBV感染者外周血细胞毒性T淋巴细胞相关抗原-4(CTLA-4)基因第1外显子区49位基因单核苷酸多态性(singlenucleotide polymorphism,SNP)与乙型肝炎病毒感染转归的关系。方法采用聚合酶链式反应-限制性片段长度多态性法检测190例慢性HBV感染者和93例既往HBV感染者外周血CTLA-4基因49位点的多态性。结果慢性HBV感染者CTLA-4基因49位点A/G基因型分布与对照组比较差异有显著性(P=0.034),慢性感染者G等位基因频率明显低于对照组(0.561对0.677,P=0.008,OR=0.607)。结论CTLA-4第1外显子49位基因多态性可能与乙型肝炎病毒感染慢性化相关。

Objective To investigate the association between the single nucleotide polymorphism of cytotoxic T lymphocyte associated agent 4（CTLA-4）gene exon 1 region（＋49）and the outcome of patients with hepatitis virus B infection. Method The restriction fragment length polymorphism-polymerase ehain reaction was used to analyze the CTLA-4 exon 1＋49 polymorphism in 190 patients with chronic hepatitis B and 93 controls with past HBV infection. Results The frequency of MG genotype at the ＋49 site was statistically different in controls and in chronic hepatitis B patients （P〈 0.05）. The frequency of G allele at the ＋49 site was statistically lower in chronie hepatitis B patients than in controls （0.561 vs 0.677,P〈0.01 ）. Conclusion The G allele of CTLA-4 exon 1＋49 polymorphism is highly related to the clinical outcomes of hepatitis B viral infection.