摘要
膜蛋白是一类在生命活动中非常重要的蛋白,但是膜蛋白三维结构的研究远远落后于水溶性蛋白.核磁共振方法由于不需要膜蛋白的晶体制备,使得该方法日益成为膜蛋白结构解析的主力.对不同的膜蛋白样品制备方法,有几种不同的核磁共振方法来进行膜蛋白的结构研究.其中液体核磁方法针对膜蛋白/去污剂胶束和膜蛋白/饼状胶束样品,并已经成功解析了多个膜蛋白结构;静态取向固体核磁共振方法针对具有均一取向的膜蛋白/饼状胶束样品和膜蛋白/磷脂双分子层样品,也成功获得了许多膜蛋白的结构信息;魔角旋转固体核磁共振方法针对膜蛋白/磷脂双分子层样品,也在发展中,并尝试着进行膜蛋白三维结构研究.
Membrane proteins play vital roles in maintaining cells proper function, but structural studies of membrane proteins lag far behind water soluble proteins. Because it is unnecessary to prepare crystals of membrane proteins, nuclear magnetic resonance （NMR） is coming to play an increasingly important role in membrane protein structural studies. Several NMR methods can be applied for different states of membrane protein samples. Solution NMR is used to study membrane protein in detergent micelles or in low q bicelles, and several membrane proteins＇ structures have been solved using solution NMR. Static oriented solid state NMR can be applied for uniformly oriented membrane proteins in high q bicelles or in lipid bilayers. Methods of magic angle spinning （MAS） solid state NMR are also under development to solve membrane protein structures in lipid bilayers.
基金
中国高技术研究发展(863)计划(2006AA02A321)资助
关键词
膜蛋白
液体核磁共振
魔角旋转固体核磁共振
静态取向固体核磁共振
membrane protein
solution NMR （nuclear magnetic resonance）
magic angle spinning solid state NMR
static oriented solid state NMR
