超临界CO_2萃取朱砂七游离蒽醌的研究

Supercritical CO_2 extraction of free anthraquinones from Polygonum Cillinerve(Nakai) Ohwi

以醋酸镁比色法测定游离蒽醌含量并以其作为评价指标,研究了超临界CO2萃取朱砂七游离蒽醌的提取工艺,探讨了萃取温度、萃取压力、萃取时间、夹带剂种类和用量、提取次数和浸泡对游离蒽醌得率的影响.结果表明,超临界萃取之前,用10 mL水浸泡朱砂七(200 mg)24h,萃取温度45℃,萃取压力35 MPa,静萃取时间35 min,夹带剂(无水乙醇)15 mL和动萃取时间30min,连续提取3次,游离蒽醌得率达3.98%,其中大黄素和大黄素甲醚分别为3.44%和0.38%.与超声法相比,超临界CO2提取工艺具有得率高、对环境友好和溶剂残留少等优点.

The supercritical CO2 fluid extraction （SFE） procedure for free anthraquinones from polygonum cillinerve （Nakai） Ohwi was studied. The contents were determined by magnesium acetate-colorimetry. The influences of extraction temperature, extraction pressure, static extraction time, volume of entrainer, maceration, and the times of repeated extracting on extracting efficiency were investigated. The results showed that the sample （200mg） should be macerated in 10 mL water for 24 h before SFE. The optimal extraction parameters were temperature 45℃, pressure 35 MPa, 15 mL of ethanol as modifiers,static extracting 35 min and dynamic extracting 30 rain, repeated extracting 3 times, respectirely. Contents of free anthraquinones,emodin and physcion obtained by SFE were 3.98%, 3. 44% and 0.38%, respectively. Compared with sonication extraction, supercritical CO2 extraction has the advantages of high yield, environment-friendly property and fewer residual solvent.