流动注射化学发光传感器测定血清中的氧氟沙星

Flow-injection chemiluminescence sensor for detemination of ofloxacin in serum

在酸性条件下,Ce（Ⅳ）氧化Ru（bipy）3^2＋生成Ru（bipy）3^3＋,同时氧化氧氟沙星生成中间态的自由基,Ru（bipy）3^3＋和中间态自由基接触后能产生强烈的化学发光.利用该反应,将Ru（bipy）3^2＋固定在阳离子交换树脂上,结合流动注射分析技术,建立了一种测定氧氟沙星的流动注射化学发光传感器.结果表明,氧氟沙星浓度在2×10^-7～8×10^-6mol/L（r=0.999 3）范围内与化学发光强度有良好的线性关系,检出限为6×10^-8mol/L（3σ）,对5×10^-6mol/L的氧氟沙星测定的相对标准偏差（RSD）为2.2%（n=11）.该传感器成功地用于血清样品中的氧氟沙星的测定.

A novel flow-injection chemiluminescence sensor for determination of ofloxacin （OFLX） is described in this paper. It was prepared by immobilizing the Ru（bipy）2^2＋ on a cation exchange resin column. OFLX and Ru（bipy）3^2＋ were oxided both by Ce（N） into Ru（bipy）3^2＋ and intermediate radical in acidic condition. Then, OFLX was sensed by the chemiluminescence（CL） reaction based on the reaction between Ru（bipy）3^2＋ and intermediate radical. The linear range of OFLX concentration is 2 × 10^-7～8 × 10^-6 mol/L（r=0.999 3）. The detection limit was 6 × 10^-8 mol/L （3δ） and the RSD was 2.2M （5 × 10^-6mol/L,n=11）. This sensor has been successfully applied to determine ofloxacin in serum.