盐度和营养限制对盐田底栖硅藻咖啡双眉藻生长及胞外多糖产率的影响

Effects of Salinity and Nutrient Limitation on the Growth and Exopolysaccharide Production of the Benthic Diatom Amphora Coffeaeformis in Salt Pan

从台南盐场藻垫中分离到硅藻咖啡双眉藻(Amphora coffeaeformis),研究了盐度和氮磷营养限制对其生长及胞外多糖(EPS)产率的影响。结果表明:高盐度和氮磷营养限制条件下咖啡双眉藻生长受到严重抑制,氮营养限制比磷营养限制对藻生长的抑制作用更显著;高盐度和氮磷营养限制条件下,咖啡双眉藻EPS产率都明显增加,氮营养限制比磷营养限制对EPS产率的促进作用更显著。这表明高盐和氮磷营养限制促进盐田中咖啡双眉藻产生更多的EPS,有利于盐田藻垫的形成和稳定,进而促进盐田藻垫防渗和净化卤水的作用。

The benthic diatom Amphora coffeaeformis was separated from the algal mat in the Tainan Saltworks. Effects of salinity and nutrient limitation on the growth and exopolysaccharide （EPS） were studied. The growth of the alga was inhibited under high salinity, nitrogen and phosphorous nutrient limitation condition. The inhibitory effect of nitrogen limitation on growth was stron- ger than phosphorous nutrient limitation. The EPS production was enhanced under high salinity, nitrogen and phosphorous nutrient limitation. The alga produced more EPS under nitrogen nutrient limitation than phosphorous and limitation. Our data suggested the alga could produce abundant EPS under high salinity and nutrient limitation in saltworks, and benefited the formation and stabilization of algal mat, and thus promoted the prevention of brine permeation and the brine purification.