摘要
目的用HPLC-ELSD法测定抗病毒分散片中薯蓣皂苷元的含量。方法采用HPLC-ELSD法,色谱柱:Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相:甲醇-水(86:14),ELSD飘移管温度88℃,载气流速2.0 mL·min^(-1)。结果薯蓣皂苷元在2.48～12.4μg范围内呈良好的线性关系(r=0.999 6),平均回收率和RSD分别为98.2%和0.5%,结论该方法简便、准确、重现性好,可作为该制剂的含量测定方法。
Objective To develop a quantitative method for determination of diosgenin in Anti - virus Dispersible Tablets by HPLC -ELSD. Methods A DiamonsilC18 column （250 mm×4. 6 mm, 5 μm） was used, methanol -water （86： 14） was used as the mobile phase, the temperature of drift tube was 88 ℃, and the flow rate of gas was 2.0 mL ·min-1. Results The calibration curves were linear between 2.48 - 12.4μg（ r = 0. 999 6）, the average recovery and the relative standard deviations were 98.2 % and O. 5 % , respectively. Conclusion The method is simple and accurate, repeatable and can be used for determination of the preparation.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第5期380-381,共2页
Traditional Chinese Drug Research and Clinical Pharmacology
