摘要
目的建立二酮前列软胶囊中黄芩素与汉黄芩素的高效液相含量测定方法。方法取出二酮前列软胶囊的内容物,用甲醇超声提取后,滤过,取滤液定容,以水-乙腈-甲醇(53:27:20)为流动相,柱温23℃,以HPLC法测定其中黄芩素与汉黄芩素的含量。结果黄芩素在6.32～31.6μg·mL^(-1)的浓度范围内,浓度对峰面积具有良好的线性关系,汉黄芩素在1.68～8.4μg·mL^(-1)的浓度范围内,浓度对峰面积具有艮好的线性关系;黄芩素平均回收率为99.58%,RSD为1.55%;汉黄芩素平均回收率为99.89%,RSD为1.61%。结论该方法简单、快捷、重复性良好,可用于二酮前列软胶囊的质量控制。
Objective To develop an HPLC method for determination of baicalein and wogonin in Ertong Qianlie Soft Capsule. Methods The contents of the Ertong Qianlie Soft Capsule were extracted by methanol and then were filtrated. The filtrate was measured by HPLC with the mobile phase of water - acetonitrile - methanol at column temperature of 23 ℃. Results The calibration curve of baicalein in the concentration range of 6.32 to 31.6 μg·mL-1 and that of wogonin in the range of 1.68 to 8.4 μg·mL-1 were in good linearity. The average recovery of baicalein was 99.58 % （ n =6, RSD I. 55 % ）, and the average recovery ofwogonin was 99.89 % （ n = 6, RSD 1.61% ）. Conclusion The method is simple, quick and reproducible, and can be applied in the quality control of Ertong Qianlie Soft Capsule.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第5期384-386,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
济南市科技计划项目(064043)。
