药用枸橼油质量标准研究

Study on Quality Standard of Citrus iimon Oils

目的建立药用枸橼油的质量标准。方法用毛细管气相色谱法对药用枸橼油中的柠檬烯进行鉴别,并用内标法测定其含量。使用聚乙二醇弹性石英毛细管柱(柱长30 m,内径0.32 mm,膜厚度0.25μm);柱温为90℃,分流比为5:1。结果含量测定方法研究表明,在0.0627 mg/mL～4.0136 mg/mL的浓度范围内,Y= 1.53321X-0.05275,r=0.999 9,柠檬烯平均回收率为97.2%,RSD为0.6%。结论该方法稳定可靠,为建立药用枸橼油质量标准提供充分依据。

Objective To develop a quality standard of Citrus limon oils. Methods Limonene in Citrus limon oils was identified by capillary gas chromatography and its content was determined by internal standard method. A polyethylene glycol elastic quartz capillary column （ 30 m of length, 0.32 mm of internal diameter, 0.25 μm of film thickness） was used, column temperature being 90 ℃ and split ratio being 5 ： 1. Results In the range of 0. 0627 mg/mL- 4. 0136 mg/mL for limonene, an equation was obtained： Y = 1. 53321X-0. 05275, r =0. 999 9. The average recovery of limonene was 97.2 % with RSD being 0.6 %. Conclusion The method is stable and reliable, and can provide evi- dences for the development of quality standard of Citrus limon oils.