肺纤方对博莱霉素致肺间质纤维化大鼠肺系数与肺组织病理的影响

Influence of Feixian Formula on lung quotiety and pulmonary pathological changes in rats with bleomycin-induced pulmonary fibrosis

目的观察肺纤方对博莱霉素致肺间质纤维化大鼠肺系数和肺组织病理的影响,研究肺纤方对肺间质纤维化的治疗作用。方法雄性W istar大鼠经气管插管灌注博莱霉素(1 mg/kg)复制大鼠肺间质纤维化模型,分为假手术组、模型组、泼尼松组和肺纤方组。造模后24 h开始常规每日1次灌胃给药。造模后第14、28、45天取材,计算肺系数,检测肺组织病理,进行肺组织病理学肺泡炎与肺间质纤维化分级;对Masson染色切片进行胶原纤维的面密度和数密度分析。结果肺纤方组28 d和45 d时肺系数显著低于14 d(P<0.05),而其他各组不同时间点比较没有统计学意义。模型组、泼尼松组、肺纤方组均有肺泡炎和肺间质纤维化改变,45 d时肺纤方组的肺间质纤维化程度较模型组和泼尼松组轻。28 d时,肺纤方组肺泡炎显著低于模型组(P<0.05)。45 d时肺纤方组与模型组比较肺间质纤维化程度明显降低(P<0.05)。定量分析显示胶原纤维的面密度和数密度在45 d时肺纤方组与模型组和泼尼松组比较均显著降低(P<0.05)。结论肺纤方有一定的减轻博莱霉素致大鼠肺间质纤维化的作用,并能在较短时间内降低博莱霉素致肺间质纤维化大鼠的肺系数。

Objective To observe the influence of Feixian Formula on lung quotiety and pulmonary pathological changes in rats with bleomycin-induced pulmonary fibrosis, and study its therapeutic effect on pulmonary fibrosis. Methods The model of pulmonary fibrosis was established by perfusion of bleomycin （ 1 mg/kg） through tracheal intubation in male Wistar rats. All rats then were divided into the sham-operated group, model group, prednisone group and Feixian Formula group. After 24 hours the rats were given drugs respectively by intragastric administration once a day. On the 14^th, 28^th and 45^th day after the establishment of the model the lung quotiety was computered, the pulmonary pathological changes were detected, the alveolifis and pulmonary fibrosis were classified, and the area density and number density of collagen fibers were analyzed by using Masson section staining. Results In the Feixian Formula group the lung quotiety was lower significantly on the 28^th and 45^th day than that on the 14^th day （P 〈 0.05 ）. There was no statistical significance of other groups between different time points. In the model group, prednisone group and Feixian Formula group there were pathological changes of alveolitis and pulmonary fibrosis. On the 45^th day the pulmonary fibrosis was slighter in the Feixian Formula group than that in the model group or prednisone group. On the 28^th day the alveolitis was slighter in the Feixian Formula group than that in the model group significantly （ P 〈 0.05 ）. On the 45^th day the degree of pulmonary fibrosis was decreased significantly in the Feixian Formula group than that in the model group （P 〈 0.05）. The quantitative analysis showed that the area density and number density of collagen fibers were decreased significantly in the Feixian Formula group than that in the model group or prednisone group （P 〈 0.05 ） on the 45^th day. Conclusion Feixian Formula has some effects of relieving pulmonary fibrosis, and reducing the lung quotiety in rats with bleomycin-induced pulmonary fibrosis in a short time.