祛风通络方对大鼠系膜细胞增殖及转化生长因子β1和白细胞介素6 mRNA表达的影响

Effects of Qufeng Tongluo Recipe on proliferation of and TGF-β1 and IL-6 mRNA expressions in glomerular mesangial cells from rats

目的:观察祛风通络方对脂多糖(lipopolysaccharide,LPS)诱导的大鼠肾小球系膜细胞(mesangial cell,MC)增殖及其分泌的转化生长因子β1(transforming growth factor-beta1,TGF-β1)和白细胞介素6(interleukin-6,IL-6)mRNA表达的影响。方法:采用血清药理学方法,体外培养大鼠肾小球系膜细胞,分为正常对照组、模型组、蒙诺(福辛普利钠)组和祛风通络方组。并采用甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)法检测大鼠MC增殖情况,逆转录聚合酶链反应法检测TGF-β1和IL-6mRNA的表达。结果:与正常对照组比较,模型组大鼠肾小球系膜细胞增殖升高(P<0.05);与模型组比较,祛风通络方组大鼠肾小球系膜细胞增殖受到抑制(P<0.05),且祛风通络方作用优于蒙诺(P<0.05)。模型组系膜细胞TGF-β1 mRNA表达高于正常对照组(P<0.01);祛风通络方组和蒙诺组系膜细胞TGF-β1 mRNA表达低于模型组(P<0.01);祛风通络方组与蒙诺组比较,TGF-β1 mRNA的表达降低(P<0.01)。LPS刺激后可提高大鼠系膜细胞内IL-6 mRNA的表达,与正常对照组比较,模型组IL-6 mRNA表达上升,差异有统计学意义(P<0.01);祛风通络方组、蒙诺组与模型组比较,IL-6 mRNA表达下降(P<0.01);祛风通络方降低IL-6 mRNA表达的作用亦优于阳性对照药蒙诺(P<0.01)。结论:祛风通络方对大鼠MC增殖及其TGF-β1和IL-6 mRNA表达均有明显的抑制作用。

Objective： To investigate the effects of Qufeng Tongluo Recipe （QFTLR）, a compound of traditional Chinese herbal medicine for dispelling wind and removing obstruction in the meridians, on cell proliferation and expressions of transforming growth factor-beta1 （TGF-β1） and interleukin-6 （IL-6） mRNAs induced by lippolysaccharide in glomerular mesangial cells from rats. Methods： The method of serum pharmacology was used. A total of 32 rats were divided into normal control group, untreated group, QFTLR group and positive control group which also was named Monoprii （fosinopril sodium） group. Mesangial proliferative glomerulonephritis was induced by injection of antithymocyte serum in the rats except for the normal control group. Sera of the rats were obtained after corresponding interventions. Lipopolysaccharide-induced proliferation of rat mesangial cells （MCs） cultured in the respective serumcontaining media was detected by the method of methyl thiazolyl tetrazolium （MTT） assay, and the expressions of TGF-β1 and IL-6 mRNAs were analyzed by the method of reverse transcription polymerase chain reaction. Results： Compared with the untreated group, QFTLR showed remarkable inhibitory function on the proliferation of the mesangial cells （P〈0. 05）. The expressions of TGF-β1 mRNA in mesangial cells were increased in the untreated group, QFTLR group and Monopril group when compared with the normal control group （P〈0. 01）, but the TGF-β1 mRNA expressions in QFTLR group and in Monopril group were lower than that in the untreated group. The IL-6 mRNA expression could be increased by the LPS stimulation, and it was significantly higher in the other three groups than that in the normal control group, including the untreated group, the Monopril group and the QFTLR group （P〈0.01）. Compared with the untreated group, the expression of IL-6 mRNA was decreased byQFTLRand Monopril （P〈0.01）. QFTLR was better than Monopril in inhibiting the proliferation of the mesangial cells and decreasing the expressions of TGF-β1 and IL-6 mRNAs （P〈0. 05）. Conclusion. QFTLR has great inhibitory effect on mesangial cell proliferation and expressions of TGF-β1 and IL-6 mRNAs, which may be one of its mechanisms in postponing glomerular sclerosis.