摘要
根据ERIC聚类分析的结果,把152株灵芝属菌株(包括128株来自中国的栽培菌株及24株国外菌株)建成48个DNA池。用SRAP和ISSR引物对48个DNA池进行扩增,筛选获得4个特异性标记,回收特异性条带,经克隆测序后设计了4对SCAR引物,并通过SCAR-PCR扩增验证,从而将SRAP标记和ISSR标记均成功地转化为特异性和稳定性更好的SCAR标记;将得到的4个SCAR标记在构成DNA池的152个菌株上验证,并建立多重PCR体系,最终证实了SCAR特异标记在菌株快速检测鉴定中的可行性和可靠性。
Forty-eight DNA pools were prepared using standardized DNA extracted from 152 Ganoderma isolates （128 Chinese isolates and 24 non-Chinese isolates） delineated on the basis of ERIC-PCR data. SRAP and ISSR methodologies were used to amplify DNA from the different pools, and four specific marker bands （one SRAP and three ISSR） were selected, cloned and sequenced. After conversion into more stable and more highly specific SCAR markers, a multiplex PCR system was developed and optimized using the corresponding SCAR primers. The feasibility and reliability of adopting strain-specific SCAR markers for the rapid identification of Ganoderma strains were confirmed.
出处
《菌物学报》
CAS
CSCD
北大核心
2008年第5期707-717,共11页
Mycosystema
基金
上海市农业科学院青年科技基金[No.2006(06)]
