蜣螂对灵芝发酵菌丝体生长和三萜产物形成的影响

Effects of powdered dung beetle (Catharsius molossus) on cell growth and triterpenoid production of Ganoderma lucidum

研究了几种药用昆虫对灵芝深层培养过程中细胞生长和三萜产物形成的影响。结果表明,添加不同药用昆虫对灵芝细胞生长和细胞内三萜的形成无显著促进作用(P>0.05),但蜣螂虫粉的添加,能显著促进灵芝胞外三萜的形成(P<0.01)。在添加浓度为5g/L时,胞外三萜的产量从对照的220.2±9.7mg/L提高到304.3±11.8mg/L。结果提示药用昆虫蜣螂虫粉中含有促进灵芝胞外三萜形成的功能因子。

The effects of several medicinal insects＇ powder on cell growth and triterpenoid production of Ganoderma lucidum in submerged fermentation were studied. The results showed that the medicinal insects＇ powder at all the tested concentration had no markedly stimulatory effects on both cell growth and intracellular triterpenoid （IGT） formation of G. lucidum. However, addition of Catharsius molossus （powdered dung beetle）at 5g/L led to a significant increase in extracellular triterpenoid （EGT） production, and the EGT yield increased from 220.2 ± 9.7mg/L to 304.3 ± 11.8mg/L. These results suggests that there are certain active components responsible for EGT formation of G. lucidum in C. molossus.