摘要
目的探讨2型糖尿病(T2DM)与簇蛋白(Glu)基因多态性的关联。方法聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)和DNA测序技术分析62例T2DM患者及60例正常对照者的Glu基因外显子2和外显子5基因多态性。结果Glu基因外显子2发现1个多态位点:1963(+A);外显子5发现3个多态位点:(1)7476(-A),(2)7534(-C),(3)7521C→T。结论Glu基因外显子2和外显子5多态性在2型糖尿病组和正常对照组多态性位点基因频率分布差异有统计学意义。因此,Clu基因外显子2和外显子5多态性可能与T2DM易感性有关联。
Objective To investigate the association of clusterin （Clu） gene polymorphism with type 2 diabetes mellitus （T2DM） Methods Clu gene was screened by PCR-DGGE in 62 cases with type 2 diabetes mellitus, 60 individuals as normal control who were without history of cardiovascular, hepatic and renal disorders. Genomic DNA were amplified using GC-clamped primers covering Clu exons 2 and 5 regions. Abnormal bands DNA were sequenced. Results 1963 （＋A） polymorphism in exon 2 of the Clu gene was detected. Three novel genetic polymorphisms were found in exon 5 of Clu-.①7476 （-A） ,②7534 （- C） ,③7521 （C→T）. Conelusions These polymorphisms are significantly different between the normal control group and the type 2 diabetes mellitus group. These polymorphisms may be associated with type 2 diabetes mellitus.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2008年第8期459-461,共3页
Chinese Journal of Diabetes
基金
贵州省科技厅科研基金资助项目(19963036)
关键词
基因
簇蛋白
糖尿病
2型
多态性
Gene
Clusterin
Diabetes mellitus,type 2
Polymorphism
