正常人脂肪餐前后血脂变化与酰化刺激蛋白的关系

Association of acylation-stimulating protein with the level of blood fat after fat load in healthy subjects

目的了解正常人脂肪餐前后血液中酰化刺激蛋白(ASP)变化,探讨ASP与糖脂代谢的关系。方法取18例正常人标准脂肪餐前及餐后2h外周静脉血,测定PG、Ins、TG、TC,酶联免疫法测定ASP及FFA水平。结果餐后ASP水平稍低于餐前水平,但差异无统计学意义。相关分析结果显示,空腹ASP(FASP)与BMI、FIns、FTG、2hTG相关,2hASP与2hFFA、FIns、FTG相关,餐后TG与FIns、FFFA、FASP相关。结论血浆ASP与BMI、Ins、TG、FFA正相关。

Objective To define the difference of plasma acylation-stimulating protein（ASP） before and after oral fat load arid to explore the relation of plasma ASP with free fatty acids（FFA）, triglyceride （TG）,total cholesterol（TC）,plasma glucose（PG） and insulin（Ins）. Methods 18 healthy subjects were given oral fat load after fasting for 10-12h. Blood examples were drawn before and 2 hours after giving fat load for determining the concentration of PG, Ins, TG, TC and ASP. ASP and FFA were determined by ELISA. Results Postprandial plasma ASP concentration[（21.26 ± 5.75） nmol/L]was not significantly lower than fasting ASP [ （24.33 ± 9.51 ） nmol/L] （P〉0. 05 ）. Linear correlation analysis showed fasting plasma acylation-stimulating protein was positively correlated with BMI, Ins, fasting and postprandial plasma TG（r = 0. 3224, P=0. 0305; r= 0. 4694, P= 0. 0494; r= 0. 4339, P = 0. 0437; r= 0. 4869, P = 0. 0405;respectively）. Postprandial plasma ASP was positively correlated with fasting Ins, fasting and postprandial plasma FFA（r=0.4510,P=0.0466;r=0.4746,P=0.0466;r= 0.4510,p=0.0457; respectively） Conclusion Plasma ASP concentration is positively correlated with BMI,Ins,TG,FFA.