白藜芦醇对重症急性胰腺炎大鼠肠黏膜屏障的保护作用

Protective Effect of Resveratrol on Intestinal Mucosal Barrier in Rats with Severe Acute Pancreatitis

目的探讨白藜芦醇对重症急性胰腺炎(SAP)大鼠肠黏膜屏障功能的保护作用。方法24只成年雄性SD大鼠随机分为假手术组(SO组)、重症急性胰腺炎组(SAP组)、白黎芦醇治疗组(RES组)。各组大鼠均于制模后6h采集标本。动态浊度法检测血清内毒素含量,TUNEL法检测肠黏膜上皮细胞凋亡率,RT-PCR检测凋亡调控基因Bax、Bcl-2 mRNA表达,激光共聚焦显微镜检测肠黏膜细胞线粒体膜电位变化。结果RES组血清内毒素水平和肠黏膜细胞凋亡指数均低于SAP组(P<0.01)。RES组Bax mRNA表达低于SAP组(P<0.01),Bcl-2 mRNA表达高于SAP组(P<0.01)。RES组线粒体膜电位高于SAP组(P<0.01)。结论白藜芦醇可通过抑制肠黏膜上皮细胞凋亡,维持肠黏膜屏障的完整性,从而防止SAP时细菌和内毒素的移居。

Objective To test the effect of resvertrol on protecting the intestinal mucosal barrier in rats with severe acute pancreatitis. Methods Twenty four SD rats were randomly divided into three groups： severe acute pancreatitis without treatments （SAP group）, severe acute panereatitis with sham-operations （SO group）, and severe acute pancreatitis treated with resveratrol （RES group）. Specimen were obtained 6 hours after the severe acute pancreatitis was induced. The endotoxin level in the blood taken from portal vein was measured with turbidimetry. Apoptosis of mucosal cells was detected by TUNEL methods. The expressions of Bax and Bcl-2 mRNA in intestinal mucosal cells were determined by semi-quantitative RT-PCR. Mitochondrial membranous electric potential of intestinal mucosal cells was measured by confocal microscopy. Results The RES group had less serum endotoxin in portal vein than the SAP group （P〈0. 01）. The SAP group had higher apoptotic index of mucosal cells than the RES group （P〈0. 01）. The SAP group had higher expression of Bax mRNA in intestinal mucosal cells and lower expression of Bcl-2 mRNA than the RES group （P〈0.01）. The SAP group had lower mitochondrial membranous electric potential of intestinal mucosal cells than the REA group （P 〈 0. 01）. Conclusion Resvertrol inhibits the apoptosis of intestinal mucosa cells and protects the intestinal barrier function, which might prevent the translocation of bacteria and endotoxin in patients with severe acute pancreatitis.