盐亭食管癌高发区饮食对大鼠氧化损伤的影响

Oxidative Damages in Rats Induced by the Diet from Yanting Region with High Prevalence of Esophageal Cancers

目的探讨盐亭食管癌高发区饮食对大鼠氧化损伤的影响。方法将30只SD雄性大鼠按体质量随机分为3组,分别喂饲大鼠常规饲料、成都食管癌低发区饮食及盐亭食管癌高发区饮食,1月后处死动物,快速取出肝脏和食管组织称重、匀浆、低温离心后分离出上清液,用试剂盒分别测定大鼠肝脏和食管组织上清液中一氧化氮(NO)、丙二醛(MDA)的含量和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮合酶(NOS)的活性。结果成都食管癌低发区饮食组大鼠肝脏和食管组织中各个指标与大鼠常规饲料对照组相应结果相似,其差异没有统计学意义(P>0.05)。盐亭食管癌高发区饮食组与成都食管癌低发区饮食组相应指标相比较,前者肝组织中NO、MDA的含量和NOS活性升高(P<0.01),CAT、GSH-Px、SOD的活性降低(P<0.01);而其食管组织中仅有MDA含量升高(P<0.05)和CAT活性降低(P<0.01),其他指标的差异则没有统计学意义(P>0.05)。结论盐亭食管癌高发区饮食具有促进大鼠肝脏氧化损伤的作用,可能具有促进大鼠食管组织氧化损伤的作用。

Objective To test the effect of the diet from Yanting region, a region with high prevalence of esophagus cancers, on oxidative damages in rats. Methods Thirty male SD rats were randomly divided into three experimental groups and fed with standard diets, Yanting diets, and Chengdu diets （a region with low prevalence of esophageal cancers）, respectively. One month later, the rats were killed. The livers and esophagus of those rats were taken immediately and were weighted, homogenated, and centrifuged at a low temperature to dissociate the supernatants. The contents of NO and MDA and the activities of NOS, SOD, CAT, and GSH-Px in the supernatants were measured. Results The rats fed with Chengdu diets showed no differences with those fed with standard diets. But the rats fed with Yanting diets had significantly higher NO, MDA and NOS, and lower activities of SOD, CAT, and GSH-Px in the livers than those fed with Chengdu diets. The rats fed with Yanting diets also had higher MHA and lower CAT in the esophagus than those fed with Chengdu diets. Conclusion Yanting diets stimulate the oxidative damages of the livers and esophagus in rats.