高效阴离子色谱法分离测定蜂蜜和保健食品多糖水解产物中的单糖组成

Determination of Monosaccharides in Honey and Polysaccharide Hydrolyzate of Functional Food by High Performance Anion Exchange Chromatography

目的建立分离测定8种单糖成分的高效阴离子色谱法。方法在AminoTMPAC PA-10（2×250mm）离子色谱分离柱上,用10.0mmol/L NaOH溶液作为流动相,在Au工作电极和pH参比电极的脉冲安培检测器上,分离测定八种单糖。结果各种单糖在0.1～5.0μg/mL浓度范围内线性良好,方法检出限2～26μg/L,标准溶液测定的保留时间和峰面积相对标准偏差分别为0.34%～7.42%和0.02%～2.16%。用本法测定了蜂蜜和保健食品多糖水解产物中的单糖组分,取得较为满意的结果,平均加标回收率为81.4%～122.0%。结论该方法无需衍生,具有较高的灵敏度和精密度,适合于蜂蜜和保健食品多糖水解产物中单糖的测定。

Objective To develope a method for analysis of eight kinds of monosaecharides using high performance anion exchange chromatography. Methods AminoTM PAC PA-10 （2× 250 mm） column with a pulse amperometrie detector with an Au working electrode and an Ag/AgCl reference electrode was applied to determine the monosaceharides. Results The linear range was 0. 1-5.0 μg/mL and the detection limits were 2-26 μg/L. The relative standard derivation for retain time and area of standard solution were 0. 34%-7. 42% and 0. 02%- 2.16% respectively, The satisfactory results were obtained for analysis of honey and polysaccharide hydrolyzate of functional food with the proposed method. The average recoveries were 81. 4%-122. 0%. The method was sensitive and with high precision. Conclusion This method was available for determination of monosaccharides in honey and polysaccharide hydrolyzate of functional food.