高山杜鹃组培苗生根移栽技术研究

Study on Rooting and Transplanting Technique of Tissue Culture Seedling of the Alpine rhododendron

采用高山杜鹃组培的无根苗为材料,筛选出相对较优的壮苗生根培养基为1/2Anderson+IBA0.5 mg/L+活性炭5 g/L+蔗糖25 g/L,其生根率达90%以上;液体培养有利于早发根和根系的生长;采用"二步法"的组培苗假值育苗技术大大提高了移栽成活率和成活质量,移栽成活率可达85%以上。

With free seedlings of the alpine rhododendron as the materials,the test was screened out that the optimun strengthening seedling and rooting culture medium was 1/2Anderson ＋ IBA0.5 mg L-1 ＋ activated carbon 5g L-1 ＋ sucrose 25g L-1,the rooting rate was above 90%;liquid culture was beneficial to the growth of root system;pre-planting technique of tissue cultured seedling with two-step method greatly improved survival rate and survival quality of the transplanting,the survival rate was above 85%.