全细胞生物催化麻疯树油制备生物柴油的研究

Study on producing biodiesel fuel from Jatropha Curcas oil sources catalized by whole cell biocatalyst

利用石油醚直接浸提法抽提得到含油率达到55·84%的麻疯树籽油作为原料,采用米根霉(Rhizopus oryzae)菌株和聚氨酯泡沫制备成固定化全细胞生物催化剂,通过对全细胞生物催化法制备生物柴油的主要工艺参数进行了优化,获得最佳工艺条件为:当甲酯化反应的醇油比为6:1,转酯化温度为35℃,转酯化体系中2%～20%质量分数的含水率,菌体量相当于油质量的4%,每12h加入一次甲醇的条件下转酯化效果最好,甲酯得率达到82·29%。同时固定化全细胞生物催化剂的重复使用性达4次,具有较高的催化性能。

The Jatropha curcas oil is extracted from Jatropha curcas seeds by petroleum ether with an oil-bearing rate reaching up to 55.84% .The immobilized Rhizopus oryzae cells as whole cell catalyst are used to catalyze transesterification of Jatropha curcas oil. Effects of different transesterification parameters on the cell growth and whole cell catalyzed transesterifieation of Jatropba cureas oils for biodiesel production are studied and gotten as follows： when the ratio of methanol to Jatropha curcas oil is 6：1 in the presence of 2% - 20% of water（w/w） and 4% of whole cell catalyst（w/w） at 35℃ ,the maximum methyl ester yield can reach 82.92%. The activity of whole cells catalyst shows a methyl ester yield of 53 % at the forth batch cycle.