Fas基因对裸鼠腹腔移植结肠癌的抑制作用

Inhibition effect of Fas gene on human colon carcinoma implanted in abdominal cavity of nude mouse

目的:探讨Fas基因对裸鼠腹腔移植人结肠癌的抑制作用。方法:构建pcDNA3-Fas质粒,用脂质体法分别将Fas基因转染体外培养的人结肠癌细胞系LOVO,用Western blot法检测目的基因的表达,用MTT法检测转染Fas基因对体外培养的人结肠癌细胞生长的影响。建立裸鼠腹腔移植人结肠癌模型,将高、中、低三种不同剂量的pcDNA3-Fas质粒注射入荷瘤小鼠腹腔,观察对荷瘤小鼠生存的影响。结果:基因转染后36h,Fas组对人结肠癌LOVO细胞的抑制作用明显强于对照组(P<0.01)。与对照组相比,高、中、低3个剂量组小鼠的生存率明显提高(P<0.01);与低剂量组相比,高、中剂量组小鼠的生存率明显提高(P<0.01),而高、中剂量组小鼠的生存率无明显差异(P>0.05)。结论:Fas基因对腹腔内结肠转移癌有抑制作用,并存在剂量依赖性。

Objective：To study the inhibition effect of Fas gene on human colon carcinoma implanted in abdominal cavity of nude mouse. Methods：Plasmid pcDNA3-Fas was constructed,and the Fas gene was transfected to human colon carcinoma LOVO cells cultured in vitro by liposome methods. The expressions of target protein were detected by Western blot.The effects of transfection of AS and Fas gene on the growth of human colon carcinoma cells were detected by MTT methods.Abdominal cavity implanted human colon carcinoma model was established in nude mice.High, median and low dosage of Plasmid pcDNA3-Fas were injected into the tumor-beared mice respectively and the survival of the mice was recorded. Results：Thirty six hours after gene transfection,compared with control group,Fas group significantly inhibited the growth of human colon carcinoma LOVO line ceils （P〈0.01）. Compared with control group,the survival rate of mice in high,median and low dosage groups was significantly improved（P〈0.01）. Compared with low dosage group, the survival rate of mice in high,median dosage groups was significantly improved （P〈0.01）. There was no significant difference in survival rate of mice between high and median dosage groups （P〉0.05）.Conclusion：Fas gene has inhibition effect on human colon carcinoma metastasized in abdominal cavity and the inhibition effect is dose dependent.