肠内免疫营养对创伤Wistar大鼠肠屏障功能的影响

Effect of enteral immunonutrition on intestinal barrieral function in wound rats

目的:探讨肠内免疫营养对创伤后大鼠肠屏障功能的影响。方法:将30只Wistar大鼠随机分为3组:对照组(Control)、普通肠内营养组(EN)和肠内免疫营养组(ELN)。通过胃造瘘术建立大鼠创伤模型,分别给予不同成分的肠内营养7天,观察各组大鼠小肠黏膜形态,免疫组化检测肠黏膜CD3+、CD4+、CD8+、IgA+细胞。结果:肠内免疫营养组的感染性并发症低于普通肠内营养组和对照组(P<0.05),而小肠黏膜绒毛高度、肠腺隐窝深度、黏膜厚度及绒毛表面积高于对照组(P<0.01)。对照组和普通肠内营养组的小肠CD3+、CD4+、CD8+、IgA+细胞数量明显低于肠内免疫营养组(P<0.01、P<0.05)。结论:肠内免疫营养能较好地改善创伤后大鼠的小肠机械屏障和免疫屏障功能,促进肠屏障功能的恢复。

Objective：To explore the effect of enteral immunonutrition on intestinal barrieral function in wound rats. Methods：Thirty wistar rats were randomly divided into three groups.Rat models of wound were established by gastrostomy. One of the two enteral nutrition formulas（standard EN and immunonutrition） was given to each group respectively for 7 days. The shape of small bowel was observed, CD3^＋,CD4^＋.CD8^＋,IgA^＋ cell quantity of the small bowel mueosa were detected through immunohistoebemical technique.Results：The results showed that the incidence of complication was decreased in immunonutrition group compared with that in enteral nutrition and control group （P〈0.05）, and small bowel mucosal villous height,crypt depth,mucosal thickness and villous surface area were significantly increased in immunonutrition group compared with control group （P〈0.01）. The quantity of CD3^＋,CD4^＋,CD8^＋,IgA^＋ cell was significantly increased in immunonutrition group compared with control and enteral nutrition group （P〈0.01 ,P〈0.05）. Conclusion：Enteral immunonutrtion may effectively improve the intestine mechanical barrier function and the immune barrier function in wound rats and promote restoration of the intestinal barrier function.