牛源耐甲氧西林金黄色葡萄球菌的分离鉴定

Isolation and Identification of Cow Source Methicillin-resistant Staphylococcus aureus

从奶牛分离得到耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)。按常规方法复苏菌株,用血浆凝固酶试验鉴定SA。用KB法、琼脂筛选法、生化鉴定法(VITEK32)、聚合酶链反应等鉴定MRSA。常规分离鉴定的葡萄球菌468株,其中凝固酶阴性葡萄球菌(coagulase negative Staphylococcus,CNS)361株,检出率为77.14%,金黄色葡萄球菌(Staphylococcus aureus,SA)105株,检出率为22.44%,MRSA 36株检出率为34.29%,2株未定型,占0.42%。而MRSA在乳房炎奶样中检出率高达34.29%,表明新疆兵团部分垦区牛场MRSA已经呈蔓延趋势。

Methicillin-resistant Staphylococcus aureus （MRSA） was isolated from cows. The convetional method was used to resuscitate the strain. S. aureus was identified by plasma coagulase test, and MRSA was identified by KB method, agar screening method, biochemical method and PCR. There were 468 Staphylococcus strains were isolated,among them 361 strains were coagulase negative, up to 77. 14%, 105 strains were S. aureus, up to 22.44% ,36 strains were MRSA, up to 34.29% ,2 strains were not identified, up to 0.42%. The percentage of MRSA in cows with mastitis was as high as 34.2%. The resuts showed that the infection with MRSA spread severely in some dairy cow farms of reclamation area in Xinjiang.