外周血薄涂片中间日疟原虫寄生红细胞的形态和颜色特征

Morphologic and chromatic parameters of erythrocytes infected with Plasmodium vivax in thin peripheral blood smears

目的探讨外周血薄涂片中间日疟原虫感染红细胞的数字化形态和颜色特征。方法使用图像处理软件进行细胞分割,并分别测量正常红细胞和间日疟原虫感染红细胞的面积、长度、宽度、周长、圆度、长宽比、等效圆直径等形态参数和细胞饱和度、色度(红、绿、蓝)等颜色参数,并进行统计分析。结果正常红细胞和感染红细胞、不同感染时期红细胞之间的形态参数和颜色参数的差异有统计学意义。结论正常红细胞与间日疟原虫感染红细胞的形态和颜色参数均有差异,这为外周血薄涂片中间日疟原虫的自动识别和诊断奠定了理论基础。

Objective To explore the digital morphological and chromatic characteristics of erythrocytes infected with Plasmodium vivax. Methods The images of both normal and P. vivax infected erythrocytes were segmented and measured for morphologic parameters including area, length, breadth, perimeter, roundness, aspect ratio, equivalent circle diameter, as well as chromatic parameters including saturation and color （red, green, blue）. A statistic analysis was performed for these parameters. Results Both morphological and chromatic parameters showed high significant differenees between the normal and the infected erythroeytes, and high significant differences between the normal and the erythrocytes infected with different stages of P. vivax. Conclusion The differences mentioned above could be used as the basis for automatic identification of P. vivax in thin peripheral blood smears.