宫颈癌组织中CDC6的表达及其与HPV_(16/18)感染的相关性

Relation between expression of CDC6 and HPV_(16/18) infection in uterine cervical carcinomas

目的研究细胞分裂周期蛋白6(CDC6)在宫颈癌组织中的表达及其与人乳头瘤病毒16/18(HPV16/18)感染的相关性。方法采用免疫组织化学方法对50例宫颈癌、宫颈上皮内瘤样病变CINⅠ20例、CINⅡ-Ⅲ20例、正常宫颈组织20例进行CDC6的检测,同时采用PCR技术检测HPV16/18感染情况。结果①宫颈癌组织中CDC6的阳性率高于CIN和正常宫颈组织(P<0.05),且CDC6阳性表达率与病理分级、淋巴转移有关(均P<0.05),而与年龄分组、临床分期进展无关(均P>0.05)。②HPV16/18在正常宫颈组织、CIN和宫颈癌中的阳性率逐渐升高,两两比较均P<0.05,但阳性表达率与年龄、临床分期、病理分级、淋巴转移无关(均P>0.05)。③宫颈癌组织中CDC6与HPV16/18感染呈正相关(rs=0.386,P<0.05)。结论宫颈上皮内瘤样病变及宫颈癌组织中CDC6表达的改变可能与HPV16/18感染有关,且互相作用,共同影响CIN的发展及宫颈癌的发生。CDC6的异常表达与宫颈癌的恶变程度有关,可作为宫颈癌筛查和预后判断的有利指标。

Objective To investigate the relationship between expression of CDC6 and HPV16/18 infection in uterine cervical carcinomas. Methods Expression of CDC6 was detected in 50 cases of squamous cell carcinomas,20 cases of cervical intraepithelial neoplasia CIN Ⅱ - Ⅲ, 20 cases of CIN Ⅰ and 20 cases of normal cervical tissues by immunohistochemistry. The infection of HPV16/18 was determined by PCR. Results （1）The positive rate of CDC6 in cervical carcinomas was higher than that in CIN and normal cervical tissues（P〈0.05）. Expression of CDC6 was correlated with tumor grades and lymph node metastasis（P〈0.05）, but not with age and clinical stages（ P 〉 0.05） . （2）The positive rate of HPV16/18 had significant difference between cervical carcinomas, CIN and normal tissues（P〈0.05）, and it was not related with tumor grades, clinical stages, age and lymph node metastasis（P 〉 0.05）. （3）The expression of CDC6 was positively correlated with the expression of HPV16/18 （ rs = 0. 386, P 〈 0.05）. Conclusion The results suggest that in CIN and cervical carcinomas CDC6 expression may be associated with HPV16/18 infection, and they interact with each other and influence the progression of CIN and carcinogenesis of cervical carcinomas. Expression of CDC6 is associated with uterine cervical carcinomas,and may be an useful index for primarily screening and predicting prognosis of cervical carcinomas.