Ⅰ型胶原物理涂层新型多孔硫酸钙支架对兔桡骨缺损修复的影响

Effect of new porous calcium sulfate scaffold overlaid by type Ⅰ collagen on bone formation in rabbits

目的研究Ⅰ型胶原物理涂层新型多孔硫酸钙支架对其成骨效能的影响。方法选用27只新西兰大白兔,制成54侧双前肢15 mm的桡骨骨缺损模型,随机分为三组:A组植入新型多孔硫酸钙支架、B组植入Ⅰ型胶原物理涂层的新型多孔硫酸钙支架、C组植入自体骨。在术后4、8、12周处死动物,进行大体观察、X线检查、X线片的计算机图像分析、骨密度检测、组织学检测、组织切片的计算机图像分析、生物力学检测并对以上结果进行统计学分析。结果术后12周各组骨缺损有不同程度的愈合,由X线检查、骨密度检测、组织切片显示在各时相点骨缺损的修复B组优于A组,但较C组差。术后12周时生物力学检测显示B组三点弯曲最大应力值大于A组,但小于C组。结论Ⅰ型胶原物理涂层新型多孔硫酸钙支架能增强其成骨效能。

Objective To explore the effect of new porous calcium sulfate scaffold overlaid by type Ⅰ collagen on bone formation. Methods Fifty-four radial bones in 27 rabbits were chosen for establishing 15 mm radial bone defect models. Then the rabbits were randomly divided into three groups. The rabbits were embed with new porous calcium sulfate scaffold overlaid by type Ⅰ collagen in group B,new porous calcium sulfate scaffold in group A and autogenous bone in group C. After operation the rabbits were sacrificed at weeks 4,8,12. X-ray examination, X-ray image analysis, bone mineral density detection, histological observation, histology image analysis, vitodynamics were performed. Results The bone defects repaired in different degree in 3 groups at week 12 after operation. At each time point roentgenographic analysis, bone mineral density and histological examination showed that the repair of bone defects in group B was more rapid than in group A,but slower than in group C. Compared with group A and group C, vitodynamics detection at week 12 showed that the crushing strength had significant difference in group B. Conclusion The new porous calcium sulfate scaffold overlaid by type Ⅰ collagen has a better effect on bone formation.