HPLC法测定光合细菌菌液中辅酶Q_(10)的含量

Determination of coenzyme Q_(10) in photosynthetic bacteria culture medium by HPLC

目的建立HPLC法测定光合细菌菌液中辅酶Q10的含量。方法样品经4500 r/min离心15 min后弃去上清液,残渣用丙酮超声萃取三次,色谱柱为Diamonsil C18(200 mm×4.6 mm,i.d.5μm),流动相为甲醇∶无水乙醇(30∶70),检测波长为275 nm,柱温25℃。结果平均加样回收率为98.54%,RSD为0.51%(n=6),线性范围为60-140μg/ml(r=0.9992,n=5)。结论该法快速、简便、灵敏、准确,适合于光合细菌菌液中辅酶Q10的含量测定。

Objective To establish a method for determining the content of coenzyme Q10 in photosynthetic bacteria liquid by HPLC. Methods The sample was eentrifugated for 15 min（4 500 r/min）, and the residue was extracted with acetone by ultrasound method for three times. HPLC was performed on a Diamonsil C18 column（200 mm × 4.6 mm, i. d. 5μm） with the mixture of methanol and anhydrous ethanol（30 ： 70）as mobile phase, with the detection wavelength at 275 nm and the column temperature at 25 ℃ . Results Theaveragerecoveryratewas98.54% with RSD 0. 51% （ n = 6 ） , and the linear range was 60 -140 gg/ml （ r = 0. 999 2, n = 5）. Conclusion The method is rapid, simple, sensitive, accurate and suitable for determining the content of coenzyme Q10 in photosynthetic bacteria liquid.