人视网膜色素上皮细胞与T淋巴细胞的激活

Human retinal pigment epithelial cells and T-lymphocyte activation

目的 观察人视网膜色素上皮（RPE）细胞人类白细胞抗原（HLA）-DP、-DQ、DR抗原和CD40抗原的表达，以确定它们在免疫应答过程中的分子表达和刺激T淋巴细胞活化的能力。方法人RPE细胞分别在γ-干扰素诱导和无诱导的条件下培养，免疫组织化学染色方法测定HLA—DP、-DQ、-DR抗原和CD40抗原在RPE细胞中的表达。体外共同培养人RPE细胞和外周血单个核细胞，荧光活化细胞分类仪测定其中活化的T淋巴细胞CD69的表达。结果γ-干扰素能够诱导HLA—DP、DQ、-DR抗原表达升高和CD40在人RPE细胞上的表达，CD69阳性的T淋巴细胞，在人RPE细胞与外周血单个核细胞的共同培养系统中的含量增加。结论人RPE细胞能够激活外周血中T淋巴细胞，是一种具有免疫学特性潜能的抗原递呈细胞。

Objective To investigate the expression of Human leucocyte antigen（HLA）-DP, -DQ, DR and CD40 in human retinal pigment epithelial （RPE） cells, to determine their molecule expression in immune response process, and their abilities to stimulate T lymphocyte activation. Methods Human RPE cells were cultured with or without （ IFN respectively. Expression of HLA DP, -DQ, -DR and CD40 was measured by immunohistoehemical staining. Meanwhile, peripheral blood mononuclear cells （PBMC） were co-cultured with RPE cells in vitro, and then the expression of activated lymphocytes CD69 was measured by fluorescence activated cell sorter（FACS）. Results Expression of HLA-DP, -DQ, -DR and CD40 antigen were enhanced by Y-interferon inducement. Increasing amount of CD69 positive lymphocytes were found in the co-culture system of RPE cells and PBMC. Conclusion T-lymphoeytes in the peripheral blood were activated by human RPE cells which is antigen presenting cells with immunological characteristics potential.