小鼠Lewis肺癌胸腔移植模型的建立

Study on establishing mice model of advanced lung cancer bearing Lewis lung carcinoma

目的：通过胸腔移植的方法建立晚期肺癌的动物模型。方法：经C37 BL／6小鼠的腋后线第6肋间胸腔（胸腔模型组）或小鼠腋部皮下（皮下模型组）分别移植鼠源性Lewis肺癌细胞0．2ml（5×10^6ml^-1），观察小鼠的生存时间、小鼠体重、饲料消耗量、游泳试验、常压耐缺氧试验，大体观察胸腔、纵隔、胸水等肺癌细胞浸润和转移情况。结果：胸腔模型组小鼠中位数生存时间13．5（8～18）d，皮下模型组小鼠中位数生存时间45（25～71）d，胸腔模型组的生存期范围离散程度较皮下模型组小（P〈0．05）。病程中胸腔模型组小鼠体重下降、摄食减少等恶病质体征较皮下模型组明显（P〈0．01），胸腔模型组小鼠活动减少、睡眠时间缩短、蜷曲、毛发脱落等现象较皮下模型组多见，胸腔模型组小鼠游泳实验及耐缺氧实验时间较皮下模型组明显缩短（P〈0．01），胸腔模型组小鼠早期可出现胸水和肿瘤转移，而皮下模型组小鼠早期未见胸水和肿瘤转移。结论：胸腔移植模型可作为晚期肺癌动物模型。

Objective To establish the mice model of advanced lung cancer bearing Lewis lung carcinoma by thoracic cavity implantation. Methods The C57BL/6 mice were divided into two groups： subcutaneous implant group and thoracic cavity implant group. The subcuhuring Lewis lung cancer cells were implanted into thoracic cavity or armpit of mice to make models. We observed the survival time, the weight of the mice, the consumption of feed, the experiment of swimming and the experiment of tolerating the absence of oxygen under general pressure. The mice were killed at the 14th day for naked eye observation and pathological examination. Results The survival time of thoracic cavity implant group without therapy was 13.5（8-18 ） days. The survival time of subcutaneous implant group without therapy was 45 （ 25-71 ） days. Thoracic cavity implant group was easier to suffer from losing weight and anorexia than subcutaneous implant group. Compared with subcutaneous implant group, thoracic cavity implant group reduced activities, woke up, shed hairs and curled. （ 1 ） Weight ： the weight of thoracic cavity implant group was significantly lower than that of subcutaneous implant group. （2） Consumption of feed ： the feed consumption of thoracic cavity implant group was significantly less than that of subcutaneous implant group. （3） Experiment of swimming and experiment of tolerating the absence of oxygen under general pressure of thoracic cavity implant group were significantly less than that of subcutaneous implant group. （4）Gross anatomy： in thoracic cavity implant group, malignant pleural effusion appeared in thoracic cavity. In the meantime, the tumor extensively spread in thoracic cavity, malignant pleural effusion appeared and metastasized to other organs, such as lung, pericardium, mediastina, and liver. Conclusion Thoracic cavity implant model can be a nice animal model of advanced lung cancer.