唾液酸寡糖-壳聚糖复合物的制备及其对禽流感病毒的吸附作用

Preparation of sialyloligosaccharide-chitosan polymer and its adsorption to avian influenza virus

利用氯仿除脂、乙醇除蛋白的方法从牛初乳中提取唾液酸寡糖，并在酸性条件下，以水为介质将其与壳聚糖进行酸碱反应．合成具有良好水溶性的唾液酸寡糖壳聚糖复合物。经筛选，优化的反应条件为：壳聚糖、唾液酸寡糖质量之比为3：50．温度控制在25C，反应时间为48h，所得唾液酸寡糖-壳聚糖复合物的接枝率、接枝效率、产率分别为50％、3%、8．5％。用所得的唾液酸寡糖-壳聚糖复合物对禽流感病毒做血凝抑制试验（HI），结果显示．当唾液酸寡糖-壳聚糖复合物浓度为20～62．5μg／ml。时，其对禽流感病毒H5、H7、H9三种亚型抗原引起的血凝均有显著抑制作用。表明，唾液酸寡糖-壳聚糖复合物对禽流感病毒有明显的吸附作用。

Sialyloligosaccharide was extracted and purified from bovine colostrum. By the acid base reaction between sialyloligosaccharide and chitosan, hydrosoluble sialyloligosaccharide-chitosan polymer was synthesized under the acid condition. The mass proportion of chitosan to sialyloligosaccharide was 3 ： 50,the optimized temperature was 25 ℃,and the reaction time was 48h. The grafting ratio, grafting efficiency and yield of the sialyloligosaccharide-chitosan polymer were 50 %,3 % and 8.5 %, respectively. The hemagglutination inhibition（HI） test of the polymer on avian influenza virus（AIV） was carried out. The HI test showed that the hemagglutinations caused by three subtype-antigens（H5, H7 and Hg） of AIV were inhibited remarkably when the concentrations of sialyloligosaccharide-chitosan polymer were 20 to 62. 5 μg/mL,indicating that the polymer had good adsorption to AIV.