叶黄素对破骨细胞分化的影响

Effect of Lutein on the Differentiation of Osteoclast Induced From Bone Marrow in Mice

研究叶黄素对体外破骨细胞分化的影响.建立由骨保护素配体(RANKL)和巨噬细胞集落刺激因子(M-CSF)共同细胞因子的小鼠破骨细胞骨髓诱导体系,将不同浓度的叶黄素作用于破骨细胞.受试细胞分为对照组、叶黄素低剂量组(10-8mol/L)、叶黄素中剂量组(10-7mol/L)和叶黄素高剂量组(10-6mol/L),并设立空白对照组.7 d后取细胞玻片进行抗酒石酸酸性磷酸酶(TRAP)染色,观察破骨细胞并计数;取骨片进行甲苯胺蓝染色,光镜下统计骨吸收陷窝面积;测量抗酒石酸酸性磷酸酶(TRAP)活性以及破骨细胞表面NF-κB活化受体(RANK)mRNA表达量.诱导培养的破骨细胞形态特征明显;叶黄素中、高剂量组在细胞数量、TRAP活性上与对照组相比有明显统计学差异(P<0.05);叶黄素各剂量组在(RANK)mRNA表达量上与对照组相比有明显统计学差异(P<0.05),且呈量效关系.研究表明叶黄素可以抑制体外培养的破骨细胞分化.

Effect of lutein on the differentiation of osteoclast induced from bone marrow were studied in vitro. Mononuclear cells of mice bone marrow were incubated with DMEM containing macrophage colony stimulating factor （ M - CSF） and receptor activator of NF - KB ligand （RANKL）. The culture was treated with lutein of different concentrations （10^-8mol/L,10^-7mol/L,10^-6mol/L）, respectively. The culture cells were fixed and were stained for tartate-resistant acidic phosphatase （TRAP） after 7d. The formation of osteoclasts was quantified by counting the number of TRAP^＋ multinuclear cells. The percentage of bone resorbed surface, TRAP activity and the expression of receptor activator of NF - KB （RANK） mRNA were analysed. The appearance of reduced mice osteoclasts was typical. Compared with the control group, the number of TRAP^＋ muhinuclear cells, TRAP activity and the percentage of bone surface in lutein middle-dose group and lutein high-dose group significantly decreased（P 〈0. 05）. But the expression of RANK mRNA significantly increased（P 〈 0. 05 ）by treated in different doses of lutein. Lutein can inhibit osteoclasts differentiation in vitro.