利用微卫星标记检测金定鸭小群保种效果

Analysis on Conserved Effect of Small Population in Jinding Duck Using Microsatellite Markers

以国家水禽种质资源基因库保存的金定鸭为素材,用12个微卫星标记对3个世代小群保种群进行遗传检测,通过分析世代间遗传差异来检测小群保种效果。结果表明：12个微卫星标记在金定鸭3个世代保种群中共检测到66个等位基因,其中60个为3个世代保种群所共有,2个为0世代所特有,各座位平均等位基因数在5个以上;3个世代的平均杂合度在0.7129~0.7298之间,平均多态信息含量在0.6658~0.6871之间,反映了金定鸭群体遗传多样性丰富,2个指标随世代增加呈现下降趋势。结果表明金定鸭采用小群保种方法是可行的。

The study was based on Jinding duck which was conserved in National Waterfowl Germplasm Resource Pool.The conserved effect was detected through comparing the difference of genetic diversity which was detected by 12 microsatellite markers in small populatin of three generations.The results suggested that 66 alleles were detected at 12 microsatellite loci in conserved population of three generations in Jinding duck.60 alleles of the total were all detected in three generations,and 2 alleles were detected only in the first generation.The number of average alleles at each locus was more than 5.The mean heterozygosity of conserved population of three generations ranged from 0.712 9 to 0.729 8,and the mean polymorphism information content ranged from 0.665 8 to 0.687 1.The genetic diversity of Jinding duck was rich.The tendency was decrease following the raise of generation.The study showed that the small population conserved method in Jinding duck was feasible.