孕酮对体外培养的牛子宫内膜细胞上孕酮受体表达的影响

Effects of Progesterone on Progesterone Receptor Expression in Cultured Bovine Endometrial Cells (bEC)

本试验分别用50、100、200和400 ng/mL浓度的孕酮处理体外培养的牛子宫内膜细胞1、3、6、12、24 h,然后收集细胞通过半定量RT-PCR分析孕酮对子宫内膜细胞孕酮受体(PR)表达的影响。结果表明:在细胞层次水平上,孕酮能够抑制子宫内膜体外培养细胞上孕酮受体的表达。

In the studies, bovine uterinal endometrium cells were cultured in medium including progesterone at 50, 100, 200 and 400 ng/mL respectively and collected after incubation for 1, 3, 6, 12 and 24 hours. Semi-quantitative reverved-transcript polymerase chain reaction （RT-PCR） analysis was used to observe the effects of progesterone on progesterone receptor （PR） expression. Results of the present study indicate that progesterone inhibit the expression of PR in vitro.