液体蛋氨酸羟基类似物对肉仔鸡生产性能和免疫反应的影响

Effects of Liquid DL-Methionine Hydroxy Analogue on Growth Performance and Immune Responses in Broiler Chickens

本试验研究了不同添加剂量的液态蛋氨酸羟基类似物(Liquid DL-Methionine Hydroxy Analogue,LMA)对肉仔鸡生产性能和免疫反应的影响。试验采用单因子4个水平的随机设计,256只1日龄AA肉公雏被随机分为4个处理,每个处理8个重复,每个重复8只鸡,分别满足肉仔鸡各阶段蛋氨酸需要量的80%、100%、120%和140%。试验对肉仔鸡生产性能、细胞免疫和体液免疫指标分别进行了测定。结果表明:随日粮LMA添加水平的提高,肉仔鸡体增重和采食量无显著变化(P>0.05),料重比呈线性降低(P<0.05),80%处理组最高(P<0.05);21日龄脾脏相对质量120%处理组显著高于80%处理组(P<0.05);血清球蛋白呈线性提高(P<0.05);外周血淋巴细胞吞噬活性呈二次曲线变化,且80%处理组显著低于其它组(P<0.05);LPS刺激的外周血淋巴细胞转化率呈二次曲线变化,其中21日龄的120%组和42日龄的100%组显著高于其它处理组(P<0.05);NDV一免后第4天和BSA二免后第13天的抗体滴度呈二次曲线变化,且100%或120%处理组显著高于其它组(P<0.05)。试验表明:日粮添加LMA可以提高肉仔鸡的饲料利用率、体液免疫和非特异性免疫活性。

An experiment was conducted to determine the effects of different doses of Liquid DLMethionine Hydroxy Analogue （LMA） on growth performance and immune response in broiler chickens. In a single factorial arrangement with 4 levels of LMA to meet 80%, 100%, 120%, 140% of methionine requirements of broiler recommended by Feeding Standard of China （2004）, 256 one-day-old Arbor Acres male broiler chickens were randomly divided into 4 treatments with 8 replicates of 8 birds each. The growth performance, cellular and hormonal immunity were respectively determined. The results showed that, with the LMA level increased, there were no significant changes （P〉0.05） in body weight gain and feed intake among the treatments, the ratio of feed to gain was linearly decreased and it was significantly highest （P〈0.05） in the group fed with 80% of methionine requirement; the relative weight of the spleen on day 21 were higher significantly （P〈0.05） in the group fed with 120% of methionine requirement than that in the 80% group; the serum globulin on day 21 and 42 were linearly increased significantly （P〈0.05） the phagocytosis of neutral red of peripheral blood lymphocyte was quadratic and the lowest wasin the control group （P〈0.05） ; the proliferation of peripheral blood lymphocyte in response to LPS and those of the 120% group on day 21 and of the 100% group on day 42 were significantly higher than the others （P〈0.05） ; the antibody titers to NDV on day 4 post first inoculation of the vaccine was quadratically increased, and the anti-BSA antibody production on day 13 post second immunization was quadratic and the hormonal immunity were highest in the groups fed with 100% or 120% of methionine requirement. In conclusion, dietary LMA supplementation im proves the feed utilization and humoral and non-specific immuno-competence of broiler chickens.