CyclinB2 mRNA在结直肠肿瘤中的表达及临床意义

Expression and signification of CyclinB2 mRNA in colorectal cancer

目的通过检测CyclinB2(细胞周期蛋白B2)在结直肠肿瘤中的表达,探讨CyclinB2与结直肠肿瘤临床病理特征的关系。方法采用逆转录酶链反应(Reverse Transcription Chain Reaction,RT-PCR)方法检测CyclinB2 mRNA在10例癌旁结直肠黏膜组织和36例结直肠肿瘤组织中的表达水平。结果CyclinB2 mRNA阳性表达率在结直肠肿瘤组织和癌旁肠黏膜组织中有明显差异(P<0.01);在低分化组中的表达水平高于高、中分化组(P<0.05);在转移组肿瘤中表达水平高于无转移组(P<0.05);在Duke D期中的表达水平明显高于A+B和C期(P<0.01);并且与患者性别、年龄、肿瘤发生部位及肿瘤大小无关。结论CyclinB2 mRNA在结直肠肿瘤组织中过度表达,可作为肿瘤恶性生物行为的判断指标。

Objective To investigate the expression of CyclinB2 in the colorectal carcinoma,and to analyze the correlation of the expression of cyclinB2 and the clinicopathologie in the eoloreetal carcinoma. Methods The different expression of CyclinB2 between the 10 non-cancerous colorectal mucous membrane tissues and 36 colorectal carcinoma tissues was detected by Reverse Transcription Chain Reaction （RT-PCR）. Results There was significan difference between the positive expression of the non-cancerous colorectal mucous and the colorectal carcinoma tissues （ P 〈 0.01 ）. The expression of CyclinB2 mRNA in the poor-dirrerentiated grade group was higher than that in the well and moderate groups （P 〈 0.05 ） ; and the expression in the metastasis group was more than that in non-metastasis ones （P 〈 0.05 ） and higher in the Dukes D group than that in Dukes A ＋ B and C（P 〈0.01 ）. There was no relationship between the expression of CyclinB2 in sex,age,tumor position and the volum of tumor. Conclusion The overexpression of CyclinB2 could act as an index to discriminate the malignant characteristic of colorectal carcinoma.