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ZBTB5基因及其表达特征的初步研究 被引量：1

A preliminary investigation about characteristics of the sequence of ZBTB5 gene and its expression
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摘要 目的了解ZBTB5基因的序列和表达特征。方法运用生物信息学方法分析该基因的序列特征和染色体定位,分子生物学方法分析其表达特征和亚细胞定位。结果ZBTB5基因的N末端含一个BTB结构域,C末端含2个C2H2锌指结构域;根据人类基因组序列数据库搜索,将ZBTB5基因定位在人类基因组第9染色体上。Northern杂交结果表明,ZBTB5基因在不同组织中表达差异明显。免疫胶体金技术显示该蛋白定位于细胞核。结论该基因可能是一个转录因子。 Objective ： To understand characteristics of the sequence of ZBTB5 gene and its expression. Methods ： The full-length cDNA sequence of ZBTB5 gene was analyzed with regard to their characteristics and localized in chromosome by bioinformatics methods. The patterns of the expression and subcellular localization were also analyzed using molecular biology methods. Results： This gene contained the characteristic N-terminal BTB domain and two C-terminal C2 H2 zinc fingers. ZBTB5 gene was mapped on chromosome 9 on the basis of the search in the human genome sequence database. Northern blotting assay indicated that ZBTB5 was expressed statistically different in the tissues. Immuno-gold labeling with the antibody against ZBTB5 fusion protein indicated that it was localized in the nuclei of K562 cell line. Conclusion ： ZBTB5 gene may be a transcriptional factor.
作者 王炎钦 姜玉新
机构地区 皖南医学院生物化学教研室 皖南医学院人体寄生虫学教研室
出处 《泰山医学院学报》 CAS 2008年第6期401-405,共5页 Journal of Taishan Medical College
基金 皖南医学院中青年基金资助项目(WK200701)
关键词 BTB结构域 生物信息学 转录因子 定位 BTB domain bioinformatics transcriptional factor localization
分类号 Q785 [生物学—分子生物学] Q786 [生物学—分子生物学]
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参考文献22

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同被引文献22

  • 1Zollman S, Godt D, Prive GG, et al. The BTB domain, found primarily in zinc finger proteins, defines an evolutionarily con- served family that includes several developmentally regulated genes in Drosophila[J1. Proc Nail Acad Sci USA, 1994, 91 (22): 10717 - 10721.
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  • 3Albagli O, Dhordain P, Deweindt C, et al. The BTB/POZ do- main : a new protein-protein interaction motif common to DNA-and actin-hinding proteins [J]. Cell Growth Differ, 1995, 6 ( 9 ) : 1193 - 1198.
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  • 7Barna M, Hawe N, Niswander L, et al. The BTB/POZ domain: a new protein-protein interaction motif common to DNA[J]. Nat Genet, 2000, 25(2): 166-172.
  • 8Bilic I, Ellmeier W. The role of BTB domain-contain/ng zinc fin- ger proteins in T cell development and function[J]. Immunol Lett, 2007, 108(1) : 1 -9.
  • 9Aravind L, Koonin EV. Fold prediction and evolutionary analysis of the POZ domain: structural and evolutionary relationship with the potassium channel tetramerization domain [J]. J Mol Bid, 1999, 285(4) : 1353 - 1361.
  • 10Chen Z, Brand NJ, Chen A, et al. Fusion between a novel Kruppel-like zinc finger gene and the retinoic acid receptor-alpha locus due to a variant t( 11 ; 17 ) translocafion associated with acute promyelocytic leukaemia[J]. EMBO J, 1993, 12(3): 1161- 1167.

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泰山医学院学报
2008年 第6期

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