辅酶Q_(10)生产菌株粟酒裂殖酵母原生质体制备和再生研究

Protoplast Preparation and Regeneration of Coenzyme Q_(10) Producing Strain Schizosaccharomyces pombe

研究对辅酶Q10生产菌株粟酒裂殖酵母原生质体制备和再生条件进行了优化,确定了原生质体制备及再生的最佳条件为:菌龄20 h,蜗牛酶浓度2 g/L,酶溶液pH值6.5,酶解温度25℃,酶解时间2 h。通过对粟酒裂殖酵母原生质体制备及再生条件的研究,建立了制备粟酒裂殖酵母原生质体的方法,以期进一步通过原生质体诱变获得辅酶Q10高产菌株。

The best condition for protoplast preparation and regeneration of Coenzyme Q10 producing strain Schizosaccharomyces pombe were studied. The optimal condition for protoplast and regeneration were confirmed to be as follows： cell age was 20 h, the content of snail enzyme was 2 g/L, the pH value of enzyme system was 6.5, euzymolysis temperature was 25 ℃, enzymolysis time was 2 hours. Based on this study, the method of the preparation and regeneration of Schizosaccharomyces pombe was developed in order to screen Coenzyme Q10 high-yielding mutant strains by mutation of the protoplasts.