密骨方对去势后骨质疏松性骨折模型大鼠骨痂BMP-3mRNA表达的研究

Research of Expression of BMP-3mRNA of Migufang on the Osteoporotic Fractures Model Rats with the Bone Densifying Method

目的应用荧光定量逆转录聚合酶链反应(RT-PCR)检测密骨方在去势后骨质疏松性骨折模型大鼠骨折愈合过程中的骨形态发生蛋白(BMP)-3mRNA水平。方法建立Wistar大鼠去势后骨质疏松性骨折的动物模型和BMP-3mRNA表达水平的SYBR GreenⅠ荧光定量RT-PCR方法,分别于术后第2、4、6周取断端组织,通过荧光定量RT-PCR检测标本中BMP-3mRNA水平。结果大鼠骨组织BMP-3mRNA表达水平:在时间上的总体比较呈递减的趋势并有显著性差异(F=153.19,P<0.05);组别的总体比较是模型组<实验组<对照组,并有显著性差异(F=262.23,P<0.05);在时间与组别的交互比较也存在显著性差异(F=81.50,P<0.05);任两组间都有统计学差异(P<0.05)。结论中药密骨方对去势后骨质疏松性骨折大鼠模型的骨折愈合有促进作用。

Objective： To test the BMP- 3mRNA level produced by Migufang in the process of healing castrated osteoporotic fractures model rats, using the Fluorescent Quantity RT - PCR. Method： Establish the animal model of the Wistar rats castrated osteoporotic fractures and the SYBR GREEN 1 Fluorescent Quantity RT- PCR method at BMP- 3Mrna expressing level. Intercept the end organization in the second, the forth and the sixth week respectively after operation. Test the BMP -3m RNA level in the samples by Fluorescent Quantity R, T -PCR. Results： The rats bone organization BMP -3m RNA expressing level： The whole comparison in the time version tends to descend little by little and has notable differences （F = 153. 19. P 〈 0. 05）. The whole comparison in the group version is that the model group is weaker than the experiment group and the experiment group is weaker than the comparison group, and has notable differences （ F = 262. 23. P 〈 0. 05）. The intersecting comparison between the time version and the group version also has notable differences （ F = 81.50. P 〈 0.05）. The comparison between any two groups has statistics differences （P 〈 O. 05）. Conclusion： The Chinese medicine Migufang can help to promote the healing of castrated osteoporotic fractures rats model.