辛夷挥发油类成分气相色谱-质谱仪指纹图谱研究

Studies on the GC-MS Fingerprint Spectrum of Essential Oil in Magnolia Biondii Pamp.

目的建立辛夷挥发油成分气相色谱-质谱（GC—MS）指纹图谱。方法采用水蒸气蒸馏法提取辛夷挥发油，用GC—MS进行成分分析。色谱柱：HP-5MS（50m×0．20mm×0．33μm）石英毛细管柱；载气：99．9％氦气；载气流速：1．0ml／min；质谱扫描范围：30～550amu。结果建立了辛夷挥发油类化学成分GC—MS指纹图谱，标定了19个共有指纹特征峰，方法学考察符合指纹图谱技术要求。结论本法可为辛夷药材质量控制提供科学依据。

Objective： To establish the GC - MS fingerprint spectrum of essential oil in Magnolia biondii Pamp.. Method： Essential oil was extracted by steam distillation （ SD ） , the chemical components of essential oil were analyzed by GC - MS. The GC - MS conditions with HP - 5MS column （50m×0.20mm×0.33μm） was used, the hold air consisted of 99. 999% He, with gradient elution at flow rate 1.0ml/min and the mass spectrometry was set at 30 -550amu. Results： 19 common characteristic peaks are taken as fingerprint peaks the precision and retest are in accordance with requirement. Conclusion： This is the first time to adopt GC - MS to analysis the chemistry set of Magnolia biondii Pamp. volatile oil cent, provided science basis for further research development of Magnolia biondii Parnp..