HPLC法测定三黄清火片中盐酸小檗碱的含量

Determination of Content of Berberine Hydrochloride in Sanhuang Qinghuo Capsules by HPLC

目的建立三黄清火片中盐酸小檗碱含量的测定方法。方法采用高效液相色谱法，色谱柱：YWG—C18（10μm，250×4．6mm），柱温：30℃；流动相：乙腈-0．025molL／L磷酸二氢钾-0．025mol·l^-1十二烷基硫酸钠（50：25：25）；检测波长：347nm；流速：1．0ml·min^-1；进样量：20μl：外标法计算含量。结果盐酸小檗碱在0．2032—2．4372μg范围内具有良好的线性关系（r=0．9990），平均回收率为99，2％，RSD为1．14％，得线性回归方程为Y=67245X＋27706（r=0．9990）。结论本方法操作简便、结果准确、重现性好，可作为该制剂盐酸小檗碱的含量测定方法。

Objective： To establish the method of determining the content of barbering hydrochloride in Sanhuang Qinghuo capsules. Method： Use HPLC. The column was YWG- C18 （10μm, 250 ×4.6mm） , the column temperature was 30℃ ; the mobile phase was acetonitrile, the potassium dihydrogent phosphate was 0. 025mol/L, the sodium dodecyl sulfate was （50 ： 25 ： 25）, the detection wavelength was 347nm; the velocity was 1. 0ml ·min^-1; the injection volume was 20μl. ReSUlts： The barbering hydrochloride has a good linear relationship from 0. 2032 to 2. 4372μg, （ r = 0. 9990） , the average recovery is 99. 2% , RSD is 1.14% , the regression equation is Y = 67245X ＋ 27706 （r = 0. 9990）. Conclusion： This method is simple , accurate,and has good reproducibility, therefore, it can be used as the method of determining the content of barbering hydrochloride.