PAI-2基因多态性与冠心病的相关性研究

Association between PAI-2 gene polymorphism and coronary heart disease

目的：探讨PAI-2基因多态性与汉族家族性冠心病的关系。方法：采用聚合酶链反应一限制性片段长度多态性分析（PCR—RFLP）对57例汉族家族性冠心病病人（冠心病组）以及62名汉族无冠心病家族史的健康人（对照组）的PAI-2基因型和等位基因频率分布进行分析，研究它与冠心病的相关性。结果：PAI-2基因Ser／Cys^413（15588位点）G／C多态性等位基因频率在冠心病组和对照组间的分布均有显著性差异（P〈0．05）。Logistic回归分析显示：CC、GG基因型与冠心病相关，且CC是冠心病发病的危险因素（OR=2．405，95％CI：1．059～5．667），GG是冠心病发病的保护性因素（OR：0．303，95％CI：0．089～1．029）。等位基因频率的相对风险分析发现，C等位基因携带者患冠心病的风险是G等位基因的28．88倍（OR=28．88，95％CI：1．96～437．62）。结论：PAI-2基因Ser／Cys^413（15588）G／C多态性与冠心病的发病具有相关性，其中C等位基因是冠心病发病的遗传易感因素，而G等位基因是冠心病发病的保护因素。

Objective： To study the allele frequencies and genotype distribution of the PAI-2 gene in Chinese patients with coronary heart disease（CHD） , and analyze the association between the PAI-2 gene polymorphism and CHD. Methods：The polymorphism of the PAI-2 gene was analyzed by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 57 patients with CHD and familial history and 62 healthy controls without CHD or familial history to research into the influence of the gene polymorphism on CHD. Results：The distribution of the PAI-2 gene Ser/Cys^413 15588 G/C polymorphism was significantly different between the CHD group and the control group （P〈0.05）. The allele frequencies distribution between the 2 groups was also different （P〈0.05）. The relative risks of different genotypes for CHD were.. CC was a risk factor for CHD （OR = 2. 405, 95%CI：1. 059-5. 667）, GG was a protective factor for CHD（OR = 0. 303, 95% CI： 0. 089-1. 029）. The relative risk in C allele carriers was 28.88 times as high as that in G allele carriers （OR =28.88, 95% CI： 1. 96-437. 62）. Conclusions：The PAI-2 gene Ser/Cys^413 15588 G/C polymorphism is associated with CHD, the C allele is a risk factor for CHD and the G allele is a protective factor for CHD.