摘要
目的:建立HPLC测定板蓝根药材及其制剂中表告依春含量的方法,为板蓝根药材及其制剂的质量控制提供依据。方法:采用反相高效液相色谱法,色谱柱为ZORBAXSB-C18(4.6mm×150mm,5μm);流动相乙腈-水-磷酸-三乙胺(8.50:90.72:0.73:0.05);流速0.7mL·min^-1;检测波长245nm,柱温30℃。结果:表告依春含量测定的线性范围0.0204~0.3060μg(r=0.9998),平均加样回收率98.99%,RSD1.31%。结论:建立了高效液相色谱法测定板蓝根药材及其制剂中表告依春含量的方法,该方法准确、可靠,可用于板蓝根药材及其制剂的质量评价。
Objective: To establish an HPLC method for the content determination of epigoitrin in Radix Isatidis and its preparation, and to provide valuable data for quality control of Radix Isatidis and its preparation. Method: The samples were separated on a ZORBAX SB - C18 (4.6 mm × 150 mm, 5 μm) column with the mobile phase of acetonitrile-water-phosphoric acid-triethylamine (8.50: 90. 72: 0.73: 0. 05) in the flow rate of 0. 7 mL ·min^-1. The detection wavelength was set at 245 nm. Column temperature was 30 %. Result: The linear range of epigoitrin was 0. 020 44). 306 0μg ( r = 0. 999 8 ), and the average recovery was 98.99% with the RSD was 1.31% ( n = 9). Conclusion: The method for quantitation of epigoitrin in Radix Isatidis and its preparation was accurate and reliable, which can be used to evaluate the quality of Radix Isatidis and its preparation.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第18期2074-2076,共3页
China Journal of Chinese Materia Medica
基金
江苏省中医药领军人才项目(2006)
苏州市科技计划(SSY0606)