毛排钱草的化学成分及其对肿瘤细胞的细胞毒活性研究

Studies on constituents of rootsanel leaves from Desmodium blandum and their cytotoxic activity against growth of several tumor cells

目的:对毛排钱草地上部分的化学成分和所得化合物对肿瘤细胞的细胞毒活性进行研究。方法:将干燥的毛排钱草地上部分用甲醇在50℃下萃取浓缩,用水溶解后过滤,得水可溶和不可溶2部分,水可溶部分用正丁醇萃取、柱色谱分离,得化合物。通过波谱解析鉴定化合物结构。并进行了细胞毒活性研究。结果:从正丁醇萃取物分离纯化得到8个化合物,分别鉴定为N,N-二甲基色胺(1),5-甲氧基-N,N-二甲基色胺(2),柠檬酚(3),异柠檬酚(4),(Z)-1-(4-羟基-2,3-二甲氧苯基)-3-(4-羟基苯)丙烯(5),(Z)-1-(3-羟基-2,4-二甲氧苯基)-3-(4-羟基-3-甲氧基苯基)丙烯(6),原儿茶酸甲酯(7),(2R,3R)-2,3-二氢-3,5,7-三羟基-2-(4-羟基苯基)-4H-1-苯骈吡喃-4-酮(8)。结论:化合物6为毛排钱草中发现的新化合物。在肿瘤细胞生长抑制活性试验(MTT test)中,化合物2,3,6均具有一定的细胞毒活性。

Objective： To investigate the chemical constituents of Desmodium blandum and their cytotoxic activity against the growth of several tumor cells. Method： Various chromatographic techniques including silica gel, Sephadex LH - 20 column chromatography were employed for the isolation and purification of the constituents. The structures of compounds were elucidated by spectral analyses （IR, UV, NMR, MS）. Their cytotoxic activity was then studied. Result： Eight compounds were isolated from the stems of D. blandum and identified as N, N-dimethyltryptamine （1）, 5-methoxy-N, N-dimethyltryptamine （2）, citrusinol （3）, yukovanol （4）, （Z） -1-（4-hydroxy-2, 3-dimethoxyphenyl） -3-（4-hydroxyphenyl） propene （5）, （ Z）-l-（3-hydroxy-2, 4-dimethoxy-phenyl） -3-（4-hy- droxy-3-methoxy-phenyl） propene （6）, methylprotocatechuate （7）, katuranin （8）. Conclusion： Among these compounds, compound 6 was isolated from D. blandum for the first time. In the MTr test, compounds 2 and 6 exhibit cytotoxic activities against the KB cell, and compounds 3 and 6 exhibit the same activities against the HepG2 cell.