摘要
目的:研究黄连解毒汤(由黄连、黄芩、黄柏、栀子等4味中药组成的复方)的化学成分,为其药代动力学和药效动力学研究提供物质基础。方法:采用各种柱色谱方法进行分离、纯化,NMR和MS等方法进行结构鉴定。结果:从黄连解毒汤的醋酸乙酯可溶性部分得到35个化合物,其中的20个化合物分别鉴定为/3-谷甾醇(1),千层纸素A(2),汉黄芩素(3),熊果酸(4),黄芩新素Ⅰ(5),韧黄芩素I(6),黄芩新素Ⅱ(7),柠檬苦素(8),5,2’-二羟基-6,7,8,3′-四甲氧基黄酮(9),白杨素(12),黄芩苷元(17),韧黄芩素Ⅱ(19),5,7,2’-三羟基-6,8-二甲氧基黄酮(21),石虎柠檬素A(22),6,2'-二羟基-5,7,8,6'-四甲氧基黄酮(26),粘毛黄芩素Ⅲ(28),5,7,4’-三羟基-8-甲氧基黄酮(29),5,7,2’,6'-四羟基黄酮(30),汉黄芩素-7-O-β-D-葡萄糖醛酸甲酯苷(31)和胡萝卜苷(34)。结论:根据已报道4味中药化学成分的研究结果,判断所有黄酮类化合物(2,3,5~7,9,12,17,19,21,26,28~30和31)来源于黄芩;化合物8,22来源于黄柏;化合物22在黄柏中首次发现。
Objective: To study the ethyl acetate soluble constituents from the water extractive of Huanglianjiedutang decoction, which are composed of Rhizoma Coptidis, Radix Seutellariae, Cortex Phellodendri and Fructus Gardeniae, and provide substances foundation for its pharmacokinetic and pharmacodynamic investigation. Method: The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by NMR and MS techniques. Result: Thirty-five compounds were isolated, among which twenty compounds have been identified as fi-sitosterol ( 1 ), oroxylin A ( 2 ), wogonin (3), ursolic acid ( 4 ), skullcapflavone Ⅰ(5), tenaxin Ⅰ (6), skullcapflavoneⅡ (7), limonin (8), 5, 2'-dihydroxy-6, 7, 8, 3'-tetramethoxyflavone (9), chrysin (12), baicalein (17), tenaxin Ⅱ (19), 5, 7, 2'- trihydroxy-6, 8-dimethoxyflavone (21), shihulimonin A (22), 6, 2'-dihydroxy-5, 7, 8, 6'-tetramethoxyflavone (26), viscidulin Ⅲ (28), 5, 7, 4'-trihydroxy-8-methoxyflavone ( 29 ), 5, 7, 2', 6'-tetra- hydroxyflavone (30), wogonin-7-O-β-D-glucuronide methyl ester (31) and daucosterol (34). Conclusion: On the basis of reported re8ults of the chemical conztituents of Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri and Fructus Gardeniae, it was estimated that all flavonoid compounds rised from the Radix Seutellariae, and compounds 8 and 22 rised from Cortex Phellodendri. Compound 22 was identified in the Cortex Phellodendri for the first time.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第18期2080-2086,共7页
China Journal of Chinese Materia Medica
基金
国家高技术领域研究"863"计划(2002AA22343C)
关键词
黄连解毒汤
化学成分
黄酮类
柠檬苦素类
Huanglian Jiedutang
chemical constituents
flavonoids
limonoids