固定化白腐真菌降解水溶液中2,4-二氯酚的研究

Biodegradation of 2,4-Dichlorophenol from Aqueous Solution by Immobilized Phanerochaete chrysosporium

研究固定化黄孢原毛平革菌对水溶液中2,4-二氯酚(2,4-DCP)的降解效果,探讨固定化黄孢原毛平革菌处理水溶液中氯酚类污染物的可行性.结果表明,采用固定化方法处理的白腐真菌,其产酶稳定性及酶活均比游离态白腐真菌有显著提高.2,4-DCP降解效果受固定化孢子接种量、pH值、摇床转速、2,4-DCP的初始浓度和表面活性剂浓度的影响.当pH为4.5,摇床转速180r/min,培养基含有1%的Tween 80,2,4-DCP初始浓度为40mg/L时,加入10mL固定化白腐真菌孢子,2,4-DCP去除效果最好.

The biodegrading effect of 2,4-Dichlorophenol from aqueous solution by immobilized Phanerochaete chrysosporium was studied and the feasibility of using immobilized Phanerochaete chrysosporium to treat the ehlorophenots-contamination water was investigated. The results showed clearly that the stability of enzymes production and enzymes activity were remarkably improved after immobilization of the free Phanerochaete chrysosporium. The biodegrade efficiency can be influenced by immobilized spore dose, optimum pH, rotating speed, initial 2,4-DCP concentration and surfactant concentration. The biodegrading efficiency was best when pH 4.5, rotate speed 180 r/min, surfaetant Tween 80 1% w/v, the initial 2,4-DCP concentration was 40 rag/L and adding 10 mL immobilized spores.