川芎嗪对脑缺血再灌注后皮质Bax mRNA表达的影响

Effect of Ligustrazine on the Expression of Bax mRNA after Cerebral Ischemia Reperfusion

目的探讨川芎嗪对脑缺血再灌注皮质Bax mRNA表达的影响。方法采用Wistar雄性大鼠30只,随机分组。A组为正常对照组,B组为模型对照组,C组为川芎嗪治疗组。采用原位杂交与医学图像分析技术检测Bax mRNA阳性细胞数目、平均灰度值。结果大脑皮质Bax mRNA在B组阳性神经元多、着色深,平均数密度值高而平均灰度值低;A组中着色浅、阳性细胞数少,平均灰度值大,二者差异较显著(P<0.01)。C组与B组比较,Bax mRNA阳性神经元少,平均灰度值升高(P<0.05)。结论脑缺血再灌注后Bax mRNA表达增强;川芎嗪可使其表达下降,在脑缺血再灌注损伤中起到神经保护作用。

Objective To investigate the effect of ligustrazine on the expression of Bax mRNA after cerebral ischemia reperfusion in rat brains. Methods Thirty male wistar rats were randomly divided into three groups, group A ： normal control group （ n = 10）, group B： model control （n = 10）, group C： ligustrazine intervention group （n = 10）. The in situ hybridization and MIPS were used to measure the number and mean gray level of Bax mRNA positive cells in cerebral cortex of the three groups. Results The cerebral cortex Bax mRNA in group B had more positive neuron and was strained deeper. The average numerical density was high, but mean gray level was low. In group A, the expression of Bax mRNA was slreined shallowand had less positive cells, but mean gray level was higher. There was significant difference between group A and group B（P 〈 0.01 ）. Compared with group B, the number of Bax mRNA positive cells in group C was increased and mean gray level was elevated（P 〈 0.05 ）. Conclusion The expression of Bax mRNA was increased after cerebral ischemia repelfusion. Ligustrazine can down - regulate the expression of Bax mRNA and protect neron from cerebral ischemia reperpusion injury.