泸州山银花中绿原酸和总绿原酸的含量测定

Determination of Chlorogenic Acid and Total Chlorogenic Acid in LuZhou Flos Lonicerae

目的建立泸州山银花中绿原酸和总绿原酸的含量测定方法。方法采用高效液相色谱（HPLC）法测定绿原酸，并采用UV法测定总绿原酸。色谱柱为Dikma Kromasil C18柱（250mm×4．6mm，5μm）；流动相为乙腈-0．4％磷酸溶液（12：88）；流速0．8ml·min^-1；检测波长326nm；柱温30℃；进样量10μl。结果绿原酸在0．16～1．6μg范围内具有良好的线性关系（r=0．9999），平均回收率为98．75％，RSD为0．29％。结论该方法操作简便、快速、有效、灵敏、准确、具有良好的重复性和回收率，可作为山银花中绿原酸和总绿原酸的含量测定方法。

Objective To establish the method for content determaination of Chlorogenic Acid and total Chlorogenic Acid in Flos Lonicerae. Methods The content of ehlorogenic acid was detected by HPLC, and total chlorogenic acid was detected by UV. The separation was carried out on a Dikma Kromasil C18 column （250 mm × 4.6 mm, 5 μm ）. The mobile phase was aeetonitrile - 0.4% phosphoric acid,the flow rate was 0.8 ml · min ^-1, the detection wavelength was set at 326 nm, the column temperature was 30℃ and the sample size was 10 bd. Results The calibration emwe showed good linearity over the range of 0.16 - 1.6 μg（ r = 0. 999 9）. The average recovery was 98.75% with RSD was 0.29%. Conclusion This method is simple, rapid, efficient, sensitive, accurate and with good repeatability and recovery,it can be used as a quantitative analysis method for this preparation.