衍生化反相高效液相色谱法测定黄杨宁片中环维黄杨星D的含量

Determination of Cyclovirobuxine D in Huangyangning Tablets by RP-HPLC with Derivatization

目的建立反相高效液相色谱法（RP—HPLC）测定黄杨宁片中环维黄杨星D的含量。方法环维黄杨星D与异氰酸苯酯反应后，用RP—HPLC测定，色谱柱为ODS—C18柱；流动相为甲醇-水（84：16）；检测波长：234nm。结果衍生化反应在23．03～53．73μg·ml^-1浓度范围内线性良好，r=0．9998（n=5），回收率均值96．1％，重复性好。结论该法准确，可靠，专属性强，可用于黄杨宁片中环雏黄杨星D的质量控制。

Objective To develop an RP - HPLC method for quantitative determination of Cyclovirohuxine D in HuangYangNing tablets. Methods Cyelovirobuxine D reacted with a derivative reagent phenyl isocyanate in chloroform. The analysis was carried out on C18 eolumn, the mobile phase was methanol -water（86： 14） ,and the detection wavelength was at 234 nm. Results Good linearity was obtained from 23.03 μg · ml^ -1 to 53.73 μg · ml^-1 of eyelovirobuxine D derivatives with a correlation coefficient of 0.999 8. The average recovery was 96.1% , RSD = 1.5%. Conclusion The method is aeeurate, reliable and specific and earl be used for the quality control of Huangyangning tablets.